Real-time Assay of Toxic Lead in In Vivo Living Plant Tissue
نویسندگان
چکیده
A method of detecting lead was developed using square wave anodic stripping voltammetry (SWASV) with DNA-carbon nanotube paste electrode (CNTPE). The results indicated a sensitive oxidation peak current of lead on the DNA-CNTPE. The curves were obtained within a concentration range of 50 ngL(-1)-20 mgL(-1) with preconcentration time of 100, 200, and 400 sec at the concentration of mgL(-1), μgL(-1), and ngL(-1), respectively. The observed relative standard deviation was 0.101% (n = 12) in the lead concentration of 30.0 μgL(-1) under optimum conditions. The low detection limit (S/N) was pegged at 8 ngL(-1) (2.6 × 10(-8) M). Results showed that the developed method can be used in real-time assay in vivo without requiring any pretreatment and pharmaceutical samples, and food samples, as well as other materials requiring water source contamination analyses.
منابع مشابه
Cytotoxicity and Genotoxicity Evaluation of Fluorapatite/bioactive Glass Nanocomposite Foams With Two Various Weight Ratios as Bone Tissue Scaffold: an in vitro study
The optimization of biomaterials’ biodegradation rate similar to tissue regeneration, is one of the main goals of tissue engineering. However, the necessity to assess their possible toxicity is always considered. The aim of this study was cytotoxicity and genotoxicity evaluation of fluorapatite/bioactive glass (FA/BG) nanocomposite foams with two various weight ratios to determine the optimal c...
متن کاملIn Vitro Assessment of The Effects of Althaea Officinalis Root Extract on Rotavirus Multiplication
Background and Aims: Medicinal plants possess a variety of beneficial characteristics without causing substantial adverse effects. Antimicrobial activity is one of the qualities, among many others, that have been identified. Althaea officinalis is an annual plant belonging to the Malvaceae family with therapeutic qualities in both the leaves and the roots. Specifically, the effects of Althaea o...
متن کاملThe role of plant antioxidants in the synthesis of metal nanoparticles
In recent years, the number of reports of nanoparticle production using green methods has increased exponentially. Green methods of nanoparticle production are based on oxidation and reduction reactions in which metal ions are reduced to nanoparticles with the help of compounds in living organisms or their extracts, including antioxidants. The presence of biomolecules, including antioxidants in...
متن کاملQuantitative detection of chicken meat routine mislabeling in emulsion type sausages and burgers by SYBR green real time PCR assay
ABSTRACT- Today, the authenticity of meat products with less costly and desirable species has increased. Therefore and considering religious, economicalor public health concerns, proper actions should be taken to prevent such frauds. In this study, real time PCR assay was applied for rapid, sensitive and specific identification and quantification of chicken tissue in meat products. Specific pri...
متن کاملEvaluation of a new set of Real-Time PCR for Brucella detection within human and animal samples
A quantitative TaqMn Real-Time PCR assay was developed and its diagnostic value on human serum and livestock samples were evaluated. Brucella species could be distributed through communities as a biological agent. Rapid detection of biological threat agents is critical for timely therapeutic administration. Quantitative real-time PCR provides a rapid, sensitive and specific tool for molecular i...
متن کامل